|
Clone ID |
Vector |
Sequencing primer |
| Human |
KUGI
(UG-****-***)
|
pCNS
pCNS-D2
pTZ18RP1
pT7T3DPac
pME18S-FL3
|
Seq1/T7promotor
Seq1/T7promotor
18RP1
T7promotor/M13F(-21)/M13R(-40)
MESEQ
|
|
IRAU
(IRAU-**-***)
IRAT
(IRAT-**-***)
Incyte
(**-*_*-***)
|
pINCY
|
M13(-21)
M13sF(-21)/M13R(-40)/SP6/T7 promoter
M13F(-21)/M13R(-21)/T7promotor/SP6
T7promotor/M13F(-21)/M13R(-40)
M13R(-40), M13F(-40), T7 promoter
|
| Mouse |
IRAV
(IRAV-**-***)
BMAP
(Bm:***-***)
NIA
(Na:NIA****-***)
|
pT7T3D-PacI
pSPORT1
|
T7promotor/M13F(-21)/M13R(-40)
M13F(-21)/M13R(-21)/T7promotor/SP6
T7promotor/M13F(-21)/M13R(-40)
SP6/M13F(-21)/M13R(-40)/T7 promoter
|
| Primer name |
Sequence (5’>3’) |
|
Seq 1
18RP1
MESEQ
T7 promoter
SP6
M13 F (-21)
M13 R (-21)
M13 F (-40)
M13 R (-40)
|
5' - GCA GAG CTC TCT GGC TAA C - 3'
5' - TTG TGT GGA ATT GTG AGC GG - 3'
5' - TCC TCA GTG GAT GTT GC - 3'
5' - TAA TAC GAC TCA CTA TAG GG - 3'
5' - ATT TAG GTG ACA CTA TAG - 3'
5' - TGT AAA ACG ACG GCC AGT - 3'
5' - AGC GGA TAA CAA TTTC ACA C - 3'
5' - GTT TTC CCA GTC ACG AC – 3'
5' - CAG GAA ACA GCT ATG AC -3'
|
|
